Product Info Summary
SKU: | PB9938 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Collagen I/COL1A1 Antibody Picoband™
SKU/Catalog Number
PB9938
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen I/COL1A1 Antibody Picoband™ catalog # PB9938. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen I/COL1A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9938)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9938 is reactive to COL1A1 in Human
Applications
PB9938 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
130-180 kDa
Calculated molecular weight
138.941kDa
Background of COL1A1
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat skin tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse skin tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 130-180 kDa. The expected band size for Collagen I is at 139 kDa.
Click image to see more details
Figure 2. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 9. IF analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 10. IF analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 11. IF analysis of Collagen I using anti-Collagen I antibody (PB9938).
Collagen I was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)
Gene Name
COL1A1
Full Name
Collagen alpha-1(I) chain
Weight
138.941kDa
Superfamily
fibrillar collagen family
Alternative Names
Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen I/COL1A1 Antibody Picoband™ (PB9938)
Hello CJ!
PB9938 has been cited in 50 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Y,Liu JM,Huang JP,Lu KX,Sun WL,Tan JY,Li BX,Chen LL,Wu Y. Regeneration potential of decellularized periodontal ligament cell sheets combined with 15-Deoxy-Δ12,14-prostaglandin J2nanoparticles in a rat periodontal defect. Biomed Mater.2021 Mar 12.doi:10.1088/1748-605X/abee61.Epub ahead of print.PMID:33711827.
Species: Human,Rat
PB9938 usage in article: APP:IHC, SAMPLE:MANDIBLES, DILUTION:1:100
Lombardi, F.; Augello, F.R.; Palumbo, P.; Mollsi, E.; Giuliani, M.; Cimini, A.M.; Cifone, M.G.; Cinque, B. Soluble Fraction from Lysate of a High Concentration Multi-Strain Probiotic Formulation Inhibits TGF-β1-Induced Intestinal Fibrosis on CCD-18Co Cells. Nutrients 2021,13,882. https://doi.org/10.3390/nu13030882
Species: Human
PB9938 usage in article: APP:WB, SAMPLE:HUMAN INTESTINAL FIBROBLAST CELL, DILUTION:1:1000
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
PB9938 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:400
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
PB9938 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:1:100
Ketamine analog methoxetamine induced inflammation and dysfunction of bladder in rats
Uncarboxylated osteocalcin inhibits high glucose-induced ROS production and stimulates osteoblastic differentiation by preventing the activation of PI3K/Akt %u2026
Synthesis, Biological Evaluation, and Docking Studies of a Novel Sulfonamido-Based Gallate as Pro-Chondrogenic Agent for the Treatment of Cartilage
Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-?1
Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-?1/Smad pathway
Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-?1-Induced Epithelial-to-Mesenchymal Transition
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Collagen I/COL1A1 Antibody Picoband™
Question
I was wanting to use your anti-Collagen I/COL1A1 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can see on the product datasheet, PB9938 anti-Collagen I/COL1A1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
Is a blocking peptide available for product anti-Collagen I/COL1A1 antibody (PB9938)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We do provide the blocking peptide for product anti-Collagen I/COL1A1 antibody (PB9938). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-03
Question
We have seen staining in human skin. Do you have any suggestions? Is anti-Collagen I/COL1A1 antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
According to literature skin does express COL1A1. According to Uniprot.org, COL1A1 is expressed in spinal cord, spleen, brain, skin, fetal brain cortex, bone, liver, among other tissues. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2019-11-28
Question
Does anti-Collagen I/COL1A1 antibody PB9938 work for WB with liver?
K. Evans
Verified customer
Asked: 2019-09-11
Answer
According to the expression profile of liver, COL1A1 is highly expressed in liver. So, it is likely that anti-Collagen I/COL1A1 antibody PB9938 will work for WB with liver.
Boster Scientific Support
Answered: 2019-09-11
Question
I have a question about product PB9938, anti-Collagen I/COL1A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9938 anti-Collagen I/COL1A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-21
Question
I see that the anti-Collagen I/COL1A1 antibody PB9938 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-09
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Collagen I/COL1A1 antibody PB9938. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Would PB9938 anti-Collagen I/COL1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
As indicated on the product datasheet, PB9938 anti-Collagen I/COL1A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-03
Question
We are currently using anti-Collagen I/COL1A1 antibody PB9938 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
The anti-Collagen I/COL1A1 antibody (PB9938) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-15
Question
Our lab were content with the WB result of your anti-Collagen I/COL1A1 antibody. However we have seen positive staining in spleen secreted using this antibody. Is that expected? Could you tell me where is COL1A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
Based on literature, spleen does express COL1A1. Generally COL1A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL1A1 expression, here are a few articles citing expression in various tissues:
Bone, Pubmed ID: 3340531
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Skin, Pubmed ID: 4319110, 5529814
Boster Scientific Support
Answered: 2017-11-14
Question
Is this PB9938 anti-Collagen I/COL1A1 antibody reactive to the isotypes of COL1A1?
K. Patel
Verified customer
Asked: 2015-10-22
Answer
The immunogen of PB9938 anti-Collagen I/COL1A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I (1194-1218aa AGFDFSFLPQPPQEKAHDGGRYYRA), different from the related mouse and rat sequences by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-10-22
Question
Is there a BSA free version of anti-Collagen I/COL1A1 antibody PB9938 available?
P. Bhatt
Verified customer
Asked: 2015-07-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Collagen I/COL1A1 antibody PB9938 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-07-17
Question
We want to test anti-Collagen I/COL1A1 antibody PB9938 on human liver for research purposes, then I may be interested in using anti-Collagen I/COL1A1 antibody PB9938 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Anderson
Verified customer
Asked: 2014-08-27
Answer
The products we sell, including anti-Collagen I/COL1A1 antibody PB9938, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Collagen I/COL1A1 antibody PB9938. Let me know if you need anything else.
D. Taylor
Verified customer
Asked: 2014-01-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-08
Question
We ordered your anti-Collagen I/COL1A1 antibody for WB on bone in a previous project. I am using human, and I plan to use the antibody for IHC next. you antibody examining bone as well as spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
A. Collins
Verified customer
Asked: 2013-12-09
Answer
I looked at the website and datasheets of our anti-Collagen I/COL1A1 antibody and it seems that PB9938 has been validated on human in both WB and IHC. Thus PB9938 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-12-09